Coding

Part:BBa_K4367003:Design

Designed by: August Qvist   Group: iGEM22_Chalmers-Gothenburg   (2022-08-01)


vioD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 979
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 808
    Illegal SapI.rc site found at 928


Design Notes

The sequence was codon optimized for Saccharomyces cerevisiae. Overhangs for making the part moClo compatible as a type 3 moClo part.


Source

This part has been codon optimized for saccharomyces cerevisiae and ordered as synthetic DNA. The original gene is found in the Chromobacterium violaceum genome. DNA sequence converted from peptide sequence found through Genbank: https://www.ncbi.nlm.nih.gov/protein/AAD51811.1

References